2026

an archive of posts from this year

May 06, 2026
May 06, 2026 Who Are These Viruses? Taxonomy and Abundance from Viral OTUs
May 04, 2026 From Metagenome to Viral OTUs: Identifying, Filtering, and Clustering Viral Genomes
Apr 30, 2026 Building a Custom GTDBtk Database for HUMAnN3 — A Gene Catalogue Approach for Environmental Metagenomics
Apr 27, 2026 Genome Coverage — What It Is, Why It Matters, and How to Calculate It for NCBI Submission
Apr 24, 2026 What is a Metadata File — and Why Does Your Microbiome Analysis Depend on It?
Apr 22, 2026 Genome Annotation Tools — From RAST's 19% to a Complete Picture with PGAP, eggNOG, DRAM, and InterProScan
Apr 20, 2026 From QIIME 2 to R — Visualizing Your 16S Amplicon Results
Apr 16, 2026 Genome Visualization Tools — A Beginner's Guide to Seeing Your Genome
Apr 14, 2026 Co-occurrence Networks from Genome Abundance Data — A Complete R Workflow
Apr 10, 2026 Conda, Mamba, Micromamba — What's the Difference (and What You Should Actually Use)
Apr 08, 2026 Day 5 — From Counts to Biology: Normalization, DESeq2, DNA:RNA Ratios, and Ecological Interpretation
Apr 06, 2026 Day 4 — Reads Meet Reference: The Mapping & Quantification Pipeline
Apr 02, 2026 Day 3 — The Reference Problem: Your Database Defines Your Biology
Mar 31, 2026 Day 2 — Clean Your RNA : QC & Preprocessing for Metatranscriptomics
Mar 30, 2026 Day 1 — Who Is Active, Not Just Present? An Introduction to Metatranscriptomics
Mar 20, 2026 Pangenome Analysis with Anvi'o: From HPC to Publication-Ready Figures
Mar 19, 2026 Day 6 — Moving Your Data: scp, rsync, SFTP, and Globus Connect
Mar 18, 2026 Day 5 — Reproducible Pipelines: Snakemake and Nextflow
Mar 17, 2026 Day 4 — Scaling Analysis: For Loops vs SLURM Job Arrays
Mar 16, 2026 Day 3 — Running Jobs: Terminal, Bash Loops, and SLURM
Mar 13, 2026 Day 2 — Software Installation: sudo vs module load vs conda
Mar 12, 2026 Day 1 — Laptop vs HPC: Why Bioinformatics Needs Both
Mar 11, 2026 Day 8 — From Raw Data to Paper Figures: A Complete Microbiome Stats Workflow
Mar 10, 2026 Day 7 — Correlation Analysis & Mantel Test: Linking MAGs to Environment
Mar 09, 2026 Day 6 — WGCNA for Microbiome Co-Abundance Networks: From Individual MAGs to Modules
Mar 06, 2026 Day 5 — Multiple Regression for MAG Abundance: Linking Genomics to Environment
Mar 05, 2026 Day 4 — Non-Parametric Tests for Microbiome Data: Wilcoxon, Kruskal-Wallis & Effect Sizes
Mar 04, 2026 Day 3 — PERMANOVA with adonis2: Testing Real Community Differences
Mar 03, 2026 Day 2 — Constrained Ordination: RDA & dbRDA for Microbiome Community Analysis
Mar 02, 2026 Day 1 — Why Normal Statistics Fail for Microbiome Data (And What We Use Instead)
Feb 27, 2026 From Messy Subfolders to Analysis-Ready FASTA: Rename with Python + Collect with Linux find
Feb 24, 2026 Day 5: Substrate Uptake & Utilization in Size-Fractionated Estuarine Microbiomes
Feb 20, 2026 Metagenome Analysis - Graduate Course Materials
Feb 18, 2026 10-Day Metagenome Analysis Series - Complete Summary
Feb 16, 2026 Day 10: Multi-Omics Integration - Metagenomics & Metatranscriptomics
Feb 14, 2026 Day 9: Visualization & Publication - Making Data Tell Stories
Feb 13, 2026 Day 8: Workflow Wrappers & Web Platforms - Streamlining Metagenome Analysis
Feb 12, 2026 Day 7: Comparative Genomics & Statistical Analysis - Connecting Genomes to Environment
Feb 10, 2026 Day 5: Genome Annotation - Understanding Metabolic Potential
Feb 09, 2026 Day 4: Genome Dereplication & Taxonomic Classification - From MAGs to Species
Feb 06, 2026 Day 3: Genome Binning - Recovering Individual Genomes (MAGs) from Metagenomes
Feb 04, 2026 Day 2: Metagenome Assembly - Reconstructing Genomes from Short Reads
Feb 02, 2026 Day 1: Quality Control, Contamination Removal, and Read-Based Taxonomic Analysis
Jan 30, 2026 Genome-Resolved Metabolism: From Metabolic Potential to Co-Metabolism Networks
Jan 29, 2026 Size-Fractionated Microbiome Analysis — Day 4: Gene Catalog Construction & Functional Annotation
Jan 28, 2026 Bridging Clinical and Ecological ML: From Heart Disease to Soil Ecosystem Multifunctionality
Jan 26, 2026 Building Custom HUMAnN3 Databases with Struo2 and GTDB: A Realistic Guide from the Trenches
Jan 22, 2026 Visualize Your Data — Day 5: Bubble Plots in Bioinformatics
Jan 21, 2026 Visualize Your Data — Day 4: Volcano Plots in Bioinformatics
Jan 20, 2026 Visualize Your Data — Day 3: Ordination Plots (PCA, PCoA, NMDS) in Bioinformatics
Jan 19, 2026 Visualize Your Data — Day 2: Heatmaps in Bioinformatics
Jan 14, 2026 Visualize Your Data — Day 1: Box Plot vs Violin Plot in Bioinformatics
Jan 14, 2026 Whole Genome Sequencing — Day 5: Comparative & Downstream Genomic Analyses
Jan 13, 2026 Whole Genome Sequencing — Day 4: Genome Annotation & Functional Potential
Jan 12, 2026 Whole Genome Sequencing — Day 3: Taxonomy, Phylogeny & Genome Similarity
Jan 08, 2026 Whole Genome Sequencing — Day 2: Genome Assembly, Quality Assessment, and Topology
Jan 07, 2026 Whole Genome Analysis — Day 1: From Raw Reads to Clean, Assembly-Ready Data
Jan 01, 2026 Exploring the Pan-Genome with panX: A Practical Workflow for DARPA Isolates