Microbial Ecology (Wet Lab)
What I do
I integrate wet-lab microbiology with genome-informed ecological analysis—spanning environmental sampling, microbial cultivation, molecular workflows, and sequencing-ready preparation to bridge field ecology with multi-omics data.
Core Areas
Field Experience Marine • Deep Sea • Environmental Systems
- Cruise-based sampling for microbiology and molecular ecology
- Deep-sea sediment and polymetallic nodule collection
- Onboard DNA/RNA isolation and sample stabilization (when applicable)
- Physicochemical characterization of sediment and marine substrates
- Seawater filtration using cartridge and Sterivex systems
- Operation of oceanographic sampling gear (Benthic Sled, beam trawl, multi-core, bongo net)
- Salt pan sediment and water sampling
- Environmental sampling for microplastic and trace metal analyses
Microbiology & Cultivation Isolation • Characterization • Applied Screening
- Cultivation and maintenance of bacterial strains from diverse environments (marine, extreme, terrestrial)
- Enumeration and physiological characterization via plating-based methods
- Identification through polyphasic taxonomy and molecular approaches
- Screening of isolates for bioremediation and bioprospecting potential
- Staining techniques and advanced microscopy
- Lyophilization and long-term strain preservation
- Functional screening of isolates for applied environmental and industrial applications
Molecular Biology & Sequencing Preparation DNA • RNA • PCR • Library Preparation
- DNA, RNA, and protein extraction from environmental and cultured samples
- PCR workflows: nested PCR, touchdown PCR, real-time qPCR
- cDNA synthesis and reverse transcription workflows
- Cloning and vector-based techniques
- SDS-PAGE and agarose gel electrophoresis
- Quantification and quality control (NanoDrop, Qubit)
- Library preparation support for metagenomic and whole-genome sequencing