Amplicon Week — Day 6: Wrap-Up: From Reads to Insights

🌱 Amplicon Week – Day 6 Wrap-Up: From Reads to Insights

This wraps up five days of Amplicon Week 🎉

Over the past week, we’ve walked through the complete lifecycle of amplicon data analysis—from raw sequencing reads to biological interpretation.

What we covered this week

Across five focused days, we explored:

● Wet-lab concepts behind amplicon sequencing and marker choice

● End-to-end QIIME2 workflows for 16S, ITS, 18S, 12S, and COI

● Advanced visualization and statistics using Microeco in R

● Functional prediction from amplicon data (PICRUSt2, Tax4Fun2, FAPROTAX)

● Web-based microbiome exploration using easy16S

If you’ve followed along, you now have multiple complementary ways to analyze amplicon datasets:

● beginner-friendly GUIs

● reproducible command-line pipelines

● flexible R-based visualization and statistics

📂 Full Amplicon Week materials

All scripts, workflows, and documentation are openly available:

🔗 https://github.com/jojyjohn28/AmpliconWeek_2025

and corresponding blog posts are below!!!

Amplicon Week — Day 1: Introduction to 16S, ITS, 18S, 12S, and COI Metabarcoding https://jojyjohn28.github.io/blog/amplicon-day1/ Amplicon Week — Day 2: QIIME2 Setup, Importing Data & Classifier Training https://jojyjohn28.github.io/blog/amplicon-day2/ Amplicon Week — Day 3: Visualization & Ecological Analysis Using QIIME2 + Microeco (R) https://jojyjohn28.github.io/blog/amplicon-day3/ Amplicon Week — Day 4: Functional Prediction from Amplicon Data (PICRUSt2, Tax4Fun2, FAPROTAX) https://jojyjohn28.github.io/blog/amplicon-day4/ Amplicon Week — Day 5: Web-based microbiome analysis with easy16S https://jojyjohn28.github.io/blog/amplicon-day5/

This repository and blogs brings together everything covered during the week—organized by day, marker type, and analysis goal—so you can reuse and adapt the workflows for your own projects.

Thanks for following Amplicon Week 2025 — and happy microbiome exploring! 🌍🧬